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Cryptococcus Molecular Detection Kit (Real-time PCR)

  • author:admin
  • date:2019-03-12
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Overview                                              

This kit is applied for quantitative detection of the DNA of Cryptococcus in Cerebrospinal fluid (CSF). It can be used for the auxiliary diagnosis of Cryptococcus infection and monitoring the therapeutic effect of drug treatment for infected patients. 


Assay Characteristics

Name

Cryptococcus Molecular Detection Kit (Real-time PCR)

Sample type

CSF

Specification

48 sample tests/kit

Detection time

2h

Detectable range

1.0X102-1.0X108  copies

Linear range

5.0X102-5.0X106 copies

Inter assay variation

≤5%

Stability

The  kit is stable for 12 months at 2-8

Machine to operate on

Fluorescence quantitative PCR instrument with VIC and ROX fluorescence groups is applicable to 0.2ml octubule.

Interpretation of results

Ct value of sample

Positive:   ≤38

The smaller of the Ct value, the severer of infection is indicated. When the Ct value of the test result is less than the Ct value of the weak positive reaction solution, it means that the infection is severe.

Negative:   >38


Performance Index

Sensitivity

92%

Specificity

97%


Product Advantages

Convenient

Sample pretreatment simplifies nucleic acid extraction

Economic

Product can be transported at 2-8, reducing the transportation costs

Accurate

The reagent is stored in PCR tube in the form of freeze-dried powder to reduce the possibility of contamination

The reagent powder is stable at 2-8, reducing the influence of temperature on the product quality

Strictly control the experiment quality with three quality controls




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