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Invasive?Fungal?Disease?Detection

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Goldstream Aspergillus Galactomannan ELISA Test

  • author:admin
  • date:2015-06-26
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Principle

The product is an indirect competition microplate assay for the detection of Aspergillus galactomannan antigen using specific antibodies. Transferring the pre-treated mixtures of the human serum sample and the anti-galactomannan antibodies into the microplate which have been coated with the galactomannan antigen. After the microplate was incubated and washed, the enzyme labelled antibodies were added. Following a second incubation and washing, TMB substrate solution was added to form a color reaction. The absorbance value was determined by a microplate reader at 450nm wavelength. The absorbance value has a negative correlation with the content of galactomannan. Then the concentration of the galactomannan in the human serum can be calculated from the standard curve and the quantitative detection can be performed.


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Reference Values

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Assay Characteristics

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Advantages

Standardized

EIA microplate test with standard procedure

Results within 3 hours reported as index values

Convenient and Quick

Ready-to-use reagents

Early detection of galactomannan antigen

6 days before onset of clinical symptoms and 10 days before diagnosis 1

1. Mark,KA, etal. J Infect Dis 2004 Aug 1;190(3) 641-649

FSC Approved and CE Marked

Adult serum samples

BAL fluid samples

Recommended by the EORTC/MSG Consensus Group

As one of the mycological criteria for the diagnosis of invasive aspergillosis 2

2. Pauw,BD, etal.Clin Infect Dis 2008 4,p.1813-1821


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