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Goldstream Bacterial Endotoxin Test(Chromogenic Method)

  • author:admin
  • date:2015-06-26
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Overview

The Goldstream Bacterial Endotoxin Test is a highly sensitive and rapid diagnostic test that detects lipopolysaccharide (LPS) qualitatively in human serum. The assay is used to provide rapid diagnosis reference for the clinicians in identifying Gram-negative bacterial infection early in the disease process.


Principle

The assay is a protease zymogen-based chromogenic assay for the qualitative detection of lipopolysaccharide (LPS) in the serum of patients with symptoms of, or medical conditions predisposing the patient to, Gram-negative bacteria infection. The serum concentration of lipopolysaccharide (LPS), a major cell-wall component of various medically important Gram-negative bacteria, can be used as an aid in the diagnosis of Gram-negative Bacteria Infection.

lipopolysaccharide (LPS) can specifically activate Factor C and Factor B in the main reagent, and the serine protease zymogen, etc. The activated Factor B converts the inactive pro-clotting enzyme to the active clotting enzyme, which in turn cleaves pNA from the chromogenic peptide substrate, Boc-Leu-Gly-Arg-pNA, creating a chromophore pNA that absorbs at 450 nm. There are additive in the reagent which can inhibit the activation of Factor G.


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Reference Values

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Assay Characteristics

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Advantages

Standardized

Endotoxin test with standard procedure

Results within 1 hours reported as index values

Convenient and Quick

Ready-to-use reagents

Early detection of Bacterial Endotoxin

FSC Approved and CE Marked

Adult and pediatric serum samples


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